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Basics of Extracellular Vesicles

This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques.

This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and the history of EVs. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs as well as the different EV cargos (RNA, protein, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Module 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Here differential ultracentrifugation, size exclusion chromatography, density gradient, kit based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be presented.

The recommended prerequisites are college-freshman-level biology and biochemistry.
After a completed course you should be able to:
+ Discuss the nomenclature and subgroups of extracellular vesicles.
+ Describe the RNA, protein and lipid content of extracellular vesicles.
+ Describe the basic concepts about the most common isolation and characterization techniques and how these techniques are used in the EV field.
+ State the benefits and limitations of the most common isolation and characterization techniques for extracellular vesicles. + Explain the considerations that are important during the collection and isolation of EVs from different body fluid.
+ Describe the release and uptake mechanisms of extracellular vesicles

All lectures are given in English.

Each of the five modules will be followed by an exam. All exams will be in the format of multiple choice questions.

The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), University of California Irvine (USA), University of Gothenburg (Sweden) and Pohang University of Science and Technology (South Korea).

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Coursera

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University of California, Irvine

Rating 4.6 based on 24 ratings
Length 6 weeks
Effort 5 weeks, 2-3 hours/week
Starts Dec 3 (last week)
Cost $49
From University of California, Irvine via Coursera
Instructor Cecilia Lasser
Free Limited Content
Language English
Subjects Science
Tags Life Sciences Medicine And Healthcare Biology

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What people are saying

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well put together in one review

Well put together and very informative.

almost completely obscure in one review

At present the course is mediocre to bad: its educational value is low: transcripts are disgusting, they are not proofread, omitted pats of sentences, inconsistent English, they almost completely obscure, disrupt the sense of the presentation.

aunque pudieron haber in one review

Muy completo, aunque pudieron haber profundizado un poco más.

but packed full in one review

This course was really well organized and paced but packed full of a lot of really good information from great sources and leaders in the field.

consejos para las in one review

Excelente curso, reafirma los conceptos básicos, da consejos para las distintas técnicas.

critical problems arise in one review

The course instructor's describe all the critical problems arise during the lab work.

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Coursera

&

University of California, Irvine

Rating 4.6 based on 24 ratings
Length 6 weeks
Effort 5 weeks, 2-3 hours/week
Starts Dec 3 (last week)
Cost $49
From University of California, Irvine via Coursera
Instructor Cecilia Lasser
Free Limited Content
Language English
Subjects Science
Tags Life Sciences Medicine And Healthcare Biology