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Next-Generation Sequencing

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May 1, 2024 Updated May 11, 2025 25 minute read
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Next-Generation Sequencing (NGS) represents a pivotal technological advancement in the biological sciences, allowing for the rapid and cost-effective sequencing of DNA and RNA. At its core, NGS technologies break down genetic material into numerous small fragments, sequence these fragments simultaneously in a massively parallel fashion, and then reassemble these "reads" using computational tools to reconstruct the original sequence. This high-throughput approach has fundamentally changed how researchers and clinicians can investigate genomes, transcriptomes (the entirety of RNA transcripts), and epigenomes (chemical modifications to DNA).

Path to Next-Generation Sequencing

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We've curated six courses to help you on your path to Next-Generation Sequencing. Use these to develop your skills, build background knowledge, and put what you learn to practice.
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Reading list

We've selected two books that we think will supplement your learning. Use these to develop background knowledge, enrich your coursework, and gain a deeper understanding of the topics covered in Next-Generation Sequencing.
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Genomics
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Provides a detailed overview of the computational and algorithmic techniques used in NGS data analysis. It covers topics such as sequence alignment, variant calling, and genome assembly.
Genomics: The Science and Technology Behind the...
Hardcover
$$$
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Forensic Science Handbook, Volume I
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Covers the use of NGS in forensic science. It discusses topics such as DNA fingerprinting and the interpretation of DNA evidence.
Forensic Science Handbook, Volume I
Paperback
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Forensic Science Handbook, Volume I
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