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The field of molecular biology has a profound impact in life science investigation. The development of more and more sophisticated experimental techniques in molecular biology with a broad, interdisciplinary applicability have stimulated research and progress in almost all the disciplines of life science. The rationality and approach of scientific experimentations have changed and allowing revolutionizing discoveries not only in molecular biology but also in biochemistry, biophysics, biotechnology, cell biology, and genetics. Reserachers can choose suitable techniques based on their usefulness, objectives and the financial and temporal resources available to the them. Many molecular biology techniques have extensively been used in the study of gene expression.

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The field of molecular biology has a profound impact in life science investigation. The development of more and more sophisticated experimental techniques in molecular biology with a broad, interdisciplinary applicability have stimulated research and progress in almost all the disciplines of life science. The rationality and approach of scientific experimentations have changed and allowing revolutionizing discoveries not only in molecular biology but also in biochemistry, biophysics, biotechnology, cell biology, and genetics. Reserachers can choose suitable techniques based on their usefulness, objectives and the financial and temporal resources available to the them. Many molecular biology techniques have extensively been used in the study of gene expression.

Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product (mRNA) that enables it to produce end products (protein), and ultimately affect a phenotype, as the final effect.

The techniques for mRNA expression analysis are very sensitive, speedy and provide complete picture. The study of gene expression involves the comparison of mRNA populations between two samples, treated versus untreated, diseased versus healthy, stage A of development versus stage B of development. The techniques used to study mRNA expression are generally divied into hybridization-based techniques, PCR-based techniques and sequencing-based techniques.

Hybridization-based techniques employ hybridization process to detect particular sequences within a complex mixture of DNA or RNA molecules. The three most popular hybridization based techniques i.e., Northern blot, RNA-Fluorescence in situ hybridization (RNA-FISH) and DNA microarray are discussed in this course in detail.

PCR-based techniques exploit the ability of Taq polymerase from Thermus Aquaticus to amplify DNA by using oligonucleotides (primers) that are a complementary sequence to the target DNA region. Reverse transcriptase polymerase chain reaction (RT-PCR) and real time qRT-PCR fell under this category and elaborated in this course.

Sequencing-based techniques determine the exact nucleotide (A, C, G, and T) order of a given DNA fragment by using sanger method or high throughput sequencing/next generation sequencing technology. In this course, serial analysis of gene expression (SAGE) and RNA sequencing (RNA seq) are explained.

Cancer can be described as a disease of altered gene expression. It is caused by the mutations in the genes that control the way our cells function, especially how they grow and divide. Scientists are working to understand the common changes that give rise to certain types of cancer or how a modification might be exploited to destroy a tumor cell.

Oncogenes and tumor suppressor genes control the normal cell growth and any mutation in these genes lead to the development of cancer. The difference between normal cell and cancerous cell pathways can be studied by analyzing gene expression levels and the biological pathways associated with the genes involved in a cancer. Lab techniques and web servers are developed by scientists to study and compare results with the already available data on different cancers.

Targeted therapy for cancer have exploited the overexpression of a specific protein or the mutation of a gene to develop a new medication to treat disease and has given rise to the field of personalized medicine.

This course is a valuable resource for students and researchers related to molecular biology, forensic science, medical laboratory technology, biotechnology, and genetics.

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Good to know

Know what's good
, what to watch for
, and possible dealbreakers
Explores gene expression, cancer development, and personalized medicine
Provides a comprehensive overview of molecular biology techniques for gene expression analysis
Taught by instructors with expertise in molecular biology and genomics
Develops skills in analyzing gene expression data, including hybridization-based, PCR-based, and sequencing-based techniques

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Activities

Be better prepared before your course. Deepen your understanding during and after it. Supplement your coursework and achieve mastery of the topics covered in Molecular Biology: Gene Expression Techniques and Cancer with these activities:
Biochemistry and Molecular Biology Refresher
Refresh your knowledge of foundational concepts in biochemistry and molecular biology.
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  • Review lecture notes, textbooks, or online resources.
  • Attend review sessions or workshops.
  • Complete practice questions or quizzes.
Study and Note Compilation
Organize and consolidate your learning materials to enhance retention and recall.
Show steps
  • Review notes, textbooks, and other resources.
  • Create a central repository for all materials.
  • Summarize and condense key concepts.
Molecular Biology of the Cell, 6th Edition
Enhance your foundation in molecular biology by reviewing a comprehensive textbook.
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  • Read assigned chapters and take notes.
  • Review figures, diagrams, and tables.
  • Summarize key concepts in your own words.
  • Engage in discussions or create study questions based on the material.
Four other activities
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PCR Basics Exercise Problems
Master the concepts of Polymerase Chain Reaction by practicing a variety of theoretical scenarios.
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  • Form a study group or review with a peer.
  • Simulate PCR experiments to practice designing primers and calculating optimal annealing temperatures.
  • Solve hypothetical scenarios to troubleshoot common PCR problems.
Gene Expression Discussion Groups
Engage with peers to clarify concepts, share perspectives, and enhance understanding of gene expression.
Show steps
  • Join or form study groups with fellow students.
  • Discuss lecture material, case studies, and research articles related to gene expression.
  • Collaborate on assignments and projects to deepen understanding.
RNA-Seq Data Analysis Project
Develop practical skills in RNA-Seq data analysis by conducting a comprehensive project.
Browse courses on RNA Sequencing
Show steps
  • Collect and prepare RNA-Seq data.
  • Perform quality control and data preprocessing.
  • Align reads to a reference genome.
  • Quantify gene expression levels.
  • Identify differentially expressed genes.
Cancer Gene Expression Analysis Project
Apply gene expression analysis techniques to gain insights into the molecular mechanisms of cancer.
Browse courses on Cancer Biology
Show steps
  • Select a cancer type and gather relevant data.
  • Analyze gene expression profiles using statistical methods.
  • Identify potential biomarkers or therapeutic targets.
  • Present findings in a scientific report or poster presentation.

Career center

Learners who complete Molecular Biology: Gene Expression Techniques and Cancer will develop knowledge and skills that may be useful to these careers:
Molecular Biologist
Molecular Biologists study the structure, function, and regulation of biological molecules, such as DNA, RNA, and proteins. They use a variety of techniques to investigate the molecular basis of life, including gene expression analysis, protein purification, and microscopy. The Molecular Biology: Gene Expression Techniques and Cancer course provides a comprehensive overview of the techniques used in molecular biology, and it covers the fundamental principles of gene expression and the role of gene expression in cancer. This course is an excellent resource for students and researchers who are interested in pursuing a career as a Molecular Biologist.
Research Geneticist
A Research Geneticist designs, conducts, and interprets experiments to study the inheritance, variation, and function of genes. They are responsible for advancing our understanding of the genetic basis of health and disease, and for developing new treatments and therapies. The Molecular Biology: Gene Expression Techniques and Cancer course provides a strong foundation for a career as a Research Geneticist by teaching the fundamental principles of gene expression and the techniques used to study it. The course also covers the role of gene expression in cancer, which is a major area of research for Research Geneticists.
Forensic Scientist
Forensic Scientists collect, analyze, and interpret evidence to help law enforcement agencies solve crimes. They use a variety of scientific techniques, including DNA analysis, fingerprint analysis, and ballistics testing. The Molecular Biology: Gene Expression Techniques and Cancer course provides a strong foundation for a career as a Forensic Scientist by teaching the fundamental principles of gene expression and the techniques used to study it. The course also covers the role of gene expression in cancer, which is a major area of research for Forensic Scientists.
Medical Laboratory Technician
Medical Laboratory Technicians perform a variety of laboratory tests to diagnose and treat diseases. They use a variety of equipment and techniques, including microscopy, cell culture, and molecular biology techniques. The Molecular Biology: Gene Expression Techniques and Cancer course provides a strong foundation for a career as a Medical Laboratory Technician by teaching the fundamental principles of gene expression and the techniques used to study it. The course also covers the role of gene expression in cancer, which is a major area of research for Medical Laboratory Technicians.
Biotechnologist
Biotechnologists use biological systems to develop new products and processes. They work in a variety of industries, including pharmaceuticals, agriculture, and environmental science. The Molecular Biology: Gene Expression Techniques and Cancer course provides a strong foundation for a career as a Biotechnologist by teaching the fundamental principles of gene expression and the techniques used to study it. The course also covers the role of gene expression in cancer, which is a major area of research for Biotechnologists.
Genetic Counselor
Genetic Counselors provide information and support to individuals and families who are affected by genetic disorders. They help patients understand their genetic risks, make informed decisions about their health care, and cope with the emotional challenges of living with a genetic disorder. The Molecular Biology: Gene Expression Techniques and Cancer course provides a strong foundation for a career as a Genetic Counselor by teaching the fundamental principles of gene expression and the techniques used to study it. The course also covers the role of gene expression in cancer, which is a major area of research for Genetic Counselors.
Science Writer
Science Writers communicate complex scientific information to a general audience. They write for a variety of outlets, including magazines, newspapers, and websites. The Molecular Biology: Gene Expression Techniques and Cancer course provides the background knowledge on gene expression and cancer necessary for a Science Writer to accurately convey information about current research and advancements in the field to a general audience.
Science Teacher
Science Teachers teach science to students at all levels, from elementary school to college. They develop lesson plans, conduct experiments, and grade assignments. The Molecular Biology: Gene Expression Techniques and Cancer course may be useful for Science Teachers who wish to incorporate more molecular biology into their curriculum. The course provides a comprehensive overview of the techniques used in molecular biology, and it covers the fundamental principles of gene expression and the role of gene expression in cancer.
Health Educator
Health Educators promote health and wellness in communities. They develop and implement programs that teach people about healthy behaviors, such as eating a healthy diet, getting regular exercise, and avoiding tobacco and alcohol. The Molecular Biology: Gene Expression Techniques and Cancer course may be useful for Health Educators who wish to learn more about the genetic basis of cancer. The course covers the role of gene expression in cancer, and it discusses the latest research on cancer prevention and treatment.
Technical Writer
Technical Writers create documentation for a variety of products and services. They write user manuals, technical reports, and marketing materials. The Molecular Biology: Gene Expression Techniques and Cancer course may be useful for Technical Writers who need to write about molecular biology or cancer. The course provides a comprehensive overview of the techniques used in molecular biology, and it covers the fundamental principles of gene expression and the role of gene expression in cancer.
Patent Attorney
Patent Attorneys help inventors protect their intellectual property. They file patents for inventions and represent clients in patent disputes. The Molecular Biology: Gene Expression Techniques and Cancer course may be useful for Patent Attorneys who need to understand the technical details of molecular biology and cancer research. The course provides a comprehensive overview of the techniques used in molecular biology, and it covers the fundamental principles of gene expression and the role of gene expression in cancer.
Regulatory Affairs Specialist
Regulatory Affairs Specialists ensure that products meet government regulations. They work with companies to develop and implement regulatory strategies, and they represent companies in front of regulatory agencies. The Molecular Biology: Gene Expression Techniques and Cancer course may be useful for Regulatory Affairs Specialists who need to understand the regulatory landscape for molecular biology and cancer research. The course covers the ethical and legal issues surrounding molecular biology and cancer research, and it discusses the latest regulatory requirements for products that are based on molecular biology.
Science Librarian
Science Librarians help scientists find and access information. They work in a variety of settings, including universities, hospitals, and research institutions. The Molecular Biology: Gene Expression Techniques and Cancer course may be useful for Science Librarians who need to learn more about molecular biology and cancer research. The course provides a comprehensive overview of the techniques used in molecular biology, and it covers the fundamental principles of gene expression and the role of gene expression in cancer.
Science Policy Analyst
Science Policy Analysts develop and implement policies that affect science and technology. They work in a variety of settings, including government agencies, universities, and think tanks. The Molecular Biology: Gene Expression Techniques and Cancer course may be useful for Science Policy Analysts who need to understand the latest advances in molecular biology and cancer research. The course covers the ethical and legal issues surrounding molecular biology and cancer research, and it discusses the potential implications of this research for public policy.
Science Communicator
Science Communicators translate complex scientific information into language that the public can understand. They work in a variety of settings, including museums, science centers, and media outlets. The Molecular Biology: Gene Expression Techniques and Cancer course may be useful for Science Communicators who need to learn more about molecular biology and cancer research. The course provides a comprehensive overview of the techniques used in molecular biology, and it covers the fundamental principles of gene expression and the role of gene expression in cancer.

Reading list

We've selected eight books that we think will supplement your learning. Use these to develop background knowledge, enrich your coursework, and gain a deeper understanding of the topics covered in Molecular Biology: Gene Expression Techniques and Cancer.
This comprehensive textbook for molecular biology. It provides a deep understanding of the principles of molecular biology, including gene expression, and covers techniques for studying gene expression.
This textbook focuses on gene expression and covers the regulation of gene expression. It includes in-depth discussions of the techniques used to study gene expression.
This comprehensive textbook for cell biology and provides background knowledge that is helpful for understanding gene expression. It includes a detailed discussion of the techniques used to study cells.
Provides a comprehensive overview of cancer biology and covers the molecular basis of cancer. It includes a discussion of the techniques used to study cancer.
This textbook provides a broad overview of cancer biology and covers the molecular basis of cancer. It includes a discussion of the techniques used to study cancer.
Provides a comprehensive overview of cancer genomics and covers the techniques used to study cancer. It useful reference for students and researchers.
Provides a comprehensive overview of cancer and covers the molecular basis of cancer and the techniques used to study cancer.

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